Regulatory

Part:BBa_K3032029:Design

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-14)


ColE based strong LexA promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LexA DNA binding sequence was carefully combined with ColE promoter sequence in order not to affect promoter's activity.



Source

This promoter sequence is design by us combining ColE promoter with LexA strong DNA binding sequence (doi:10.1093/nar/gki939).


References